Services

What we provide?

Molecular Biology Services

  • Individual Cloning Package: gene amplification, plasmid construction, site directed mutagenesis.
  • Combo cloning packages (shuttling vectors): Flexi vector system or Gateway system.​
  • Plasmid isolations ​​

Protein Expression Services

  • Bacterial expression services (E coli auto-induction)
  • Combo cloning Yeast expression services (Pichia spp., Saccharomyces spp.)packages (shuttling vectors): Flexi vector system or Gateway system.
  • Insect cell expression (Sf9, Sf21, High Five, Shneider2 (S2))​​
  • Mammalian expression services
  • Cell-free expression services (prokaryotic and eukaryotic)

Protein Purification Services

  • Affinity Chromatography : His tag, MBP, GST fusion
  • Ion exchange (cation and anion exchange)
  • Plasmid isolationsGel Filtration and etc..,​​

Protein Labelling/ Engineering Services

  • Unusual amino acid incorporation includes selenomethionine/fluorinated amino acids
  • 15N labeling/ 13C and 15N labeling/ 15N, 13C, 2H labeling​
  • Plasmid isolations​​
  • Protein dissection – mapping, structural and functional studies

Proteins are abundant in all organisms, acting as enzymes, antibodies, hormones or structural elements. Because of their essential role in living systems, great efforts have been made to elucidate their structures in order to understand their function for drug discovery and other applications, which include industrial enzymes. A high level of protein expression and optimal purification is imperative for protein biology research groups in academia as well as industry for broad applications.

Depending on specific applications, the system chosen for protein expression can be critical for the end-point use of the desired protein.

It is a fact that each of the expression systems has its strengths and weaknesses concerning yield, proper folding, post-translational modification (PTM), cost, speed and ease of use. Hence, OxonEx Biological Pvt. Ltd. aims to establish the following expression systems as shown in Fig 1.

Fig. 1. : Expression and purification platforms within the facility

M              1               2               3

M- Marker

1- Soluble protein

2- Ni-NTA purification

3- TEV cleavage fraction

Cloning of gene into expression vector (with or without fusion tag)

Transformation into expression strain (E.coli)

Expression of recombinant protein (with
or without fusion protein)

 


 

Small scale expression. Samples were
analyzed by SDS PAGE. 

 

 


Large scale expression to obtain
required quantity of protein and downstream processing of cell paste which
includes

• Cell Lysis

• Separation of soluble fractions

• Dialysis

•Purification (affinity,
Ion-exchange chromatography and gel filtration)

• Lyophilization (if requested)