{"id":908,"date":"2023-01-30T05:21:26","date_gmt":"2023-01-30T05:21:26","guid":{"rendered":"http:\/\/oxoon.fasna.site\/?page_id=908"},"modified":"2023-11-26T16:58:53","modified_gmt":"2023-11-26T16:58:53","slug":"services","status":"publish","type":"page","link":"https:\/\/www.oxonex.com\/index.php\/services\/","title":{"rendered":"services"},"content":{"rendered":"\t\t
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Services<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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What we provide?<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMolecular Biology Services\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

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  • Individual Cloning Package: gene amplification, plasmid construction, site directed mutagenesis.<\/li>\n
  • Combo cloning packages (shuttling vectors): Flexi vector system or Gateway system.\u200b<\/li>\n
  • Plasmid isolations \u200b\u200b<\/li>\n<\/ul>\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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    \n\t\t\t\t\t\t\n\t\t\t\t\t\t\tProtein Expression Services\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

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    • Bacterial expression services (E coli auto-induction)<\/li>\n
    • Combo cloning Yeast expression services (Pichia spp., Saccharomyces spp.)packages (shuttling vectors): Flexi vector system or Gateway system.<\/li>\n
    • Insect cell expression (Sf9, Sf21, High Five, Shneider2 (S2))\u200b\u200b<\/li>
    • Mammalian expression services<\/li>
    • Cell-free expression services (prokaryotic and eukaryotic)<\/li>\n<\/ul>\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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      \n\t\t\t\t\t\t\n\t\t\t\t\t\t\tProtein Purification Services\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

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      • Affinity Chromatography : His tag, MBP, GST fusion<\/li>\n
      • Ion exchange (cation and anion exchange)<\/li>\n
      • Plasmid isolationsGel Filtration and etc..,\u200b\u200b<\/li>\n<\/ul>\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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        \n\t\t\t\t\t\t\n\t\t\t\t\t\t\tProtein Labelling\/ Engineering Services\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

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        • Unusual amino acid incorporation includes selenomethionine\/fluorinated amino acids<\/li>\n
        • 15N labeling\/ 13C and 15N labeling\/ 15N, 13C, 2H labeling\u200b<\/li>\n
        • Plasmid isolations\u200b\u200b<\/li>
        • Protein dissection \u2013 mapping, structural and functional studies<\/li>\n<\/ul>\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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          Proteins are abundant in all organisms, acting as enzymes, antibodies, hormones or structural elements. Because of their essential role in living systems, great efforts have been made to elucidate their structures in order to understand their function for drug discovery and other applications, which include industrial enzymes. A high level of protein expression and optimal purification is imperative for protein biology research groups in academia as well as industry for broad applications.<\/p>

          Depending on specific applications, the system chosen for protein expression can be critical for the end-point use of the desired protein.<\/p>

          It is a fact that each of the expression systems has its strengths and weaknesses concerning yield, proper folding, post-translational modification (PTM), cost, speed and ease of use. Hence,\u00a0OxonEx<\/b>\u00a0Biological Pvt. Ltd.\u00a0<\/b>aims to establish the following expression systems as shown in Fig 1.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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          Fig. 1. <\/b>: Expression and purification platforms within the facility<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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          M              1               2               3 <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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          M- Marker<\/p>

          1- Soluble protein<\/p>

          2- Ni-NTA purification<\/p>

          3- TEV cleavage fraction<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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          Cloning of gene into expression vector (with or without fusion tag)<\/p>

          \"\"<\/p>

          Transformation into expression strain (E.coli)<\/p>

          \"\"<\/p>

          Expression of recombinant protein (with
          or without fusion protein)<\/p>

          \u00a0<\/p>

          \"\"
          <\/span><\/p>

          \u00a0<\/p>

          Small scale expression. Samples were
          analyzed by SDS PAGE.\u00a0<\/p>

          \u00a0<\/p>

          \u00a0<\/p>

          \"\"
          <\/span><\/p>

          Large scale expression to obtain
          required quantity of protein and downstream processing of cell paste which
          includes<\/p>

          \u2022\u00a0Cell Lysis<\/p>

          \u2022\u00a0Separation of soluble fractions<\/p>

          \u2022\u00a0Dialysis<\/p>

          \u2022Purification (affinity,
          Ion-exchange chromatography and gel filtration)<\/p>

          \u2022\u00a0Lyophilization (if requested)\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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          <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

          Services What we provide? Molecular Biology Services Individual Cloning Package: gene amplification, plasmid construction, site directed mutagenesis. Combo cloning packages (shuttling vectors): Flexi vector system or Gateway system.\u200b Plasmid isolations \u200b\u200b Protein Expression Services Bacterial expression services (E coli auto-induction) Combo cloning Yeast expression services (Pichia spp., Saccharomyces spp.)packages (shuttling vectors): Flexi vector system or […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-908","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.oxonex.com\/index.php\/wp-json\/wp\/v2\/pages\/908","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.oxonex.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.oxonex.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.oxonex.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.oxonex.com\/index.php\/wp-json\/wp\/v2\/comments?post=908"}],"version-history":[{"count":140,"href":"https:\/\/www.oxonex.com\/index.php\/wp-json\/wp\/v2\/pages\/908\/revisions"}],"predecessor-version":[{"id":2220,"href":"https:\/\/www.oxonex.com\/index.php\/wp-json\/wp\/v2\/pages\/908\/revisions\/2220"}],"wp:attachment":[{"href":"https:\/\/www.oxonex.com\/index.php\/wp-json\/wp\/v2\/media?parent=908"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}